原著論文 総説・特許 講演Google Scholar, English


47. Nakamura A, Tasaki T, Okuni Y, Song C, Murata K, Kozai T, Hara M, Sugimoto H, Suzuki K, Watanabe T, Uchihashi T, Noji H, *Iino R
Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis
Phys Chem Chem Phys 2018 20: 3010-3018. DOI: 10.1039/C7CP04606E

Featured on the inside back cover!



46. #Baba M, #Iwamoto K, Iino R, Ueno H, Hara M, Nakanishia A, Kishikawa J, *Noji H, *Yokoyama K (#Equal contribution)

Rotation of artificial rotor axles in rotary molecular motors
PNAS 2016 113: 11214-11219. DOI: 10.1073/pnas.1605640113


45. Nakamura A, Tasaki T, Ishiwata D, Yamamoto M, Okuni Y, Visootsat A, Maximilien M, Noji H, Uchiyama T, Samejima M, Igarashi K, *Iino R

Single-molecule imaging analysis of binding, processive movement, and dissociation of cellobiohydrolase Trichoderma reesei Cel6A and its domains on crystalline cellulose
J. Biol. Chem. 2016 291: 22404-22413. DOI: 10.1074/jbc.M116.752048

Featured on the cover!


44. #Isojima H, #Iino R, Niitani Y, Noji H, *Tomishige M (#Equal contribution)
Direct observation of intermediate states during the stepping motion of kinesin-1
Nat Chem Biol 2016 12: 290-297. DOI: 10.1038/nchembio.2028

News and Views in Nature Chemical Biology: Motor proteins: Kinesin's gait captured



43. *Matsumoto Y, Sakakihara S, Grushnikov A, Kikuchi K, Noji H, Yamaguchi A, Iino R, Yagi Y, Nishino K
A microfluidic channel method for rapid drug-susceptibility testing of Pseudomonas aeruginosa
PLOS ONE 2016. 11(2): e0148797. DOI: 10.1371/journal.pone.0148797



42. Obayashi Y, Iino R, *Noji H
A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate
Analyst 2015. 140: 5065-73. doi: 10.1039/c5an00714c


41. Enoki S, Iino R, Niitani Y, Minagawa Y, Tomishige M, *Noji H
High-speed angle-resolved imaging of single gold nanorod with microsecond temporal resolution and one-degree angle precision
Anal. Chem. 2015 87: 2079-2086. doi: 10.1021/ac502408c


40. Yukawa A, Iino R, Watanabe R, Hayashi S, *Noji H
Key chemical factors of arginine finger catalysis of F1-ATPase clarified by an unnatural amino acid mutation
Biochemistry. 2015 54: 472–480. doi: 10.1021/bi501138b



39. #Ueno H, #Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Noji H, *Murata T, *Iino R  (#Equal contribution)
Torque generation of Enterococcus hirae V-ATPase
J. Biol. Chem. 2014 289: 31212-31223. doi: 10.1074/jbc.M114.598177


38. Ikeda T, Tsukahara T, Iino R, Takeuchi M, *Noji H
Motion capture and manipulation of single synthetic molecular rotors by optical microscopy
Angew. Chem. Int. Ed. 2014 53: 10082–10085. DOI: 10.1002/anie.201403091

プレスリリース: 1ナノメートルの人工分子マシン1個を「見て、触る」ことに成功:光学顕微鏡による 1分子モーションキャプチャ

Will be featured on the back cover!


37. Ikeda T, Iino R, *Noji H
Real-time fluorescence visualization of slow tautomerization of single free-base phthalocyanines under ambient conditions
Chem. Commun. 2014 50: 9443-9446. DOI: 10.1039/C4CC02574A

マイナビニュース記事. Introduced in UTokyo Research! (English, Japanese)
Real-time visualization of slow tautomerization in a single organic chromophore : Candidate for a 1-nanometer-sized molecular memory
Featured on the front cover!


36. Shibafuji Y, Nakamura A, Uchihashi T, Sugimoto N, Fukuda S, Watanabe H, Samejima M, Ando T, Noji H, Koivula A, Igarashi K, *Iino R
Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII
J. Biol. Chem. 2014 289: 14056-14065. doi: 10.1074/jbc.M113.546085


35. Takehara H, Miyazawa K, Noda T, Sasagawa K, Tokuda T, Kim SH, Iino R, Noji H, *Ohta J
A CMOS image sensor with stacked photodiodes for lensless observation system of digital enzyme-linked immunosorbent assay
Jpn. J. Appl. Phys. 2014 53: 04EL02. doi: 10.7567/JJAP.53.04EL02



34. #Minagawa Y, #Ueno H, Hara M, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Yamato I, Muneyuki E, Noji H, *Murata T, *Iino R (#Equal contribution)
Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase
J. Biol. Chem. 2013 288: 32700-32707. doi: 10.1074/jbc.M113.506329
Featured on the front cover! Designed by SCIEMENT


33. Fukuda S, Uchihashi T, Iino R, Okazaki Y, Yoshida M, Igarashi K, and *Ando T
High-speed atomic force microscope combined with single-molecule fluorescence microscope
Rev. Sci. Instrum. 2013 84: 073706. doi: 10.1063/1.4813280


32. Watanabe R, Tabata KV, Iino R, Ueno H, Iwamoto M, Oiki S, *Noji H
Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force
Nat. Commun. 2013 4: 1631. DOI:10.1038/ncomms2631



31. Enoki S, Iino R, Morone N, Kaihatsu K, Sakakihara S, Kato N, *Noji H
Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy
PLOS ONE 2012. 7: e49208. DOI:10.1371/journal.pone.0049208


30. Kim SH, Iwai S, Araki S, Sakakihara S, Iino R, *Noji H
Large-scale femtoliter droplet array for digital counting of single biomolecules
Lab Chip 2012. 12: 4986-4991. DOI: 10.1039/C2LC40632B


29. You H, Iino R, Watanabe R, *Noji H
Winding single-molecule double-stranded DNA on a nanometer-sized reel
Nucleic Acids Research 2012. 40: e151. doi: 10.1093/nar/gks651


28. *Iino R, Hayama K, Amezawa H, Sakakihara S, Kim SH, Matsumoto Y, Nishino K, Yamaguchi A, Noji H
A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array
Lab Chip 2012. 12: 3923-3929. DOI: 10.1039/C2LC40394C
Introduced as one of the Emerging Investigators in Lab on a Chip!


27. *Hayashi S, Ueno H, Shaikh AR, Umemura M, Kamiya M, Ito Y, Ikeguchi M, Komoriya Y, Iino R, Noji H
Molecular mechanism of ATP hydrolysis in F1-ATPase revealed by molecular simulations and single molecule observations.
J. Am. Chem. Soc. 2012. 134: 8447–8454. DOI:10.1021/ja211027m


26. Komoriya Y, Ariga T, Iino R, Imamura H, Okuno D, *Noji H
Principle role of the arginine finger in rotary catalysis of F1-ATPase
J. Biol. Chem. 2012. 287: 15134-15142. DOI: 10.1074/jbc.M111.328153


25. *Sasagawa K, Ando K, Kobayashi T, Noda T, Tokuda T, Kim SH, Iino R, Noji H, Ohta J
Complementary metal–oxide–semiconductor image sensor with microchamber array for fluorescent bead counting
Jpn. J. Appl. Phys. 2012. 51. 02BL01. DOI: 10.1143/JJAP.51.02BL01


24. #Watanabe R, #Okuno D, Sakakihara S, Shimabukuro K, Iino R, Yoshida M, *Noji H (#Equal contribution)
Mechanical modulation of catalytic power on F1-ATPase
Nat. Chem. Biol. 2012. 8: 86-92. doi: 10.1038/nchembio.715



23. #Uchihashi T, #Iino R, *Ando T, *Noji H. (#Equal contribution)
High-speed atomic force microscopy reveals rotary catalysis of rotorless F1-ATPase
Science 2011. 333: 755-758. DOI: 10.1126/science.1205510
Perspective of the Science
News of the Chemistry World
Research Highlight of the Nature Nanotech.
News of the BioTechniques



22. *Matsumoto Y, Hayama K, Sakakihara S, Nishino K, Noji H, *Iino R, Yamaguchi A.
Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels
PLOS ONE 2011.  6: e18547.



21. Okuno D, Iino R, and *Noji H.
Stiffness of γ subunit of F1-ATPase
Eur. Biophys. J. 2010. 39:1589-1596.


20. Watanabe R, Iino R, *Noji H.
Phosphate-release in F1-ATPase catalytic cycle follows ADP release.
Nat. Chem. Biol. 2010. 6: 814–820. doi:10.1038/nchembio.443

Introduced in News and Views!


19. *Hayashi K, Ueno H, Iino R, and *Noji H.
Fluctuation theorem applied to F1-ATPase
Phys. Rev. Lett. 2010. 104: 218103-1-218103-4.


18. Ueno H, Nishikawa S, Iino R, Tabata KV, Sakakihara S, Yanagida T, *Noji H.
Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution
Biophys. J. 2010. 98: 2014-2023.


17. Saita E, Iino R, Suzuki T, Feniouk BA, Kinosita K. Jr, *Yoshida M.
Activation and stiffness of the inhibited states of F1-ATPase probed by single-molecule manipulation
J Biol Chem. 2010. 285: 11411-11417.


16. Sakakihara S, Araki S, *Iino R, *Noji H.
A single-molecule enzymatic assay in a directly accessible femtoliter droplet array
Lab Chip. 2010. 10: 3355-3362.



15. *Imamura H, Huynh Nhat KP, Togawa H, Saito K, Iino R, Kato-Yamada Y, Nagai,T, *Noji H.
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
Proc. Natl. Acad. Sci. U.S.A. 2009. 106: 15651-15656.


14. Enoki S, Watanabe R, Iino R, and *Noji H.
Single-molecule study on the temperature-sensitive reaction of F1-ATPase with a hybrid F1 carrying a single β(E190D)
J. Biol. Chem. 2009. 284: 23169-23176.


13. *Iino R, Hasegawa R, Tabata KV, *Noji H.
Mechanism of inhibition by C-terminal α-helices of the ε subunit of Escherichia coli FoF1-ATP synthase
J. Biol. Chem. 2009. 284:17457-17464.



12. Okuno D, Fujisawa R, Iino R, Hirono-Hara Y, Imamura H, *Noji H.
Correlation between the conformational states of F1-ATPase as determined from its crystal structure and single-molecule rotation
Proc. Natl. Acad. Sci. U.S.A. 2008. 105: 20722-20727.


11. Watanabe R, Iino R, Shimabukuro K, Yoshida M, *Noji H.
Temperature-sensitive reaction intermediate of F1-ATPase
EMBO rep. 2008. 9: 84-90.



10. Suzuki KGN, Fujiwara TK, Sanematsu F, Iino R, Edidin M, and *Kusumi A.
GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1
J. Cell Biol. 2007. 177: 717-730.



9. Iino R, Murakami T, Iizuka S, Kato-Yamada Y, Suzuki T and *Yoshida M.
Real-time monitoring of conformational dynamics of the epsilon subunit in F1-ATPase
J. Biol. Chem. 2005. 280: 40130-40134.


8. Makiyo H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, *Yokoyama K and *Iwata S.
Structure of a central stalk subunit F of Prokaryotic V-type ATPase/synthase from Thermus thermophilus
EMBO J. 2005. 24: 3974-3983.


7. Koyama-Honda I, Ritchie K, Fujiwara T, Iino R, Murakoshi H, Kasai RS, and *Kusumi A.
Fluorescence imaging for monitoring the colocalization of two single molecules in living cells
Biophys. J. 2005. 88: 2126-2136.



6. Murase K, Fujiwara T, Umemura Y, Suzuki K, Iino R, Yamashita H, Saito M, Murakoshi H, Ritchie K, and *Kusumi A.
Ultrafine membrane Compartments for molecular diffusion as revealed by single molecule techniques
Biophys. J. 2004. 86: 4075-4093.


5. Murakoshi H, Iino R, Kobayashi T, Fujiwara T, Ohshima C, Yoshimura A, and *Kusumi A.
Single-molecule imaging analysis of ras activation in living cells
Proc. Natl. Acad. Sci. U.S.A. 2004. 101: 7317-7322.



4. Suzuki T, Murakami T, Iino R, Suzuki J, Ono S, Shirakihara Y, and *Yoshida M.
FoF1-ATPase/synthase is geared to the synthesis mode by conformational rearrangement of epsilon subunit in response to proton motive force and ADP/ATP balance
J. Biol. Chem. 2003. 278: 46840-46846.


3. Nakada C., Ritchie K., Oba Y, Nakamura M, Hotta Y, Iino R, Kasai S, Yamaguchi K, Fujiwara T, and *Kusumi A.
Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization
Nat. Cell Biol. 2003. 5: 626-632.


2. Ike H, Kosugi A, Kato A, Iino R, Hirano H, Fujiwara T, Ritchie K, and *Kusumi A.
Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single molecule fluorescence video imaging
ChemPhysChem. 2003. 4: 620-626.



1. Iino R, Koyama I, and *Kusumi A.
Single molecule imaging of green fluorescent protein in living cells: E-cadherin forms oligomers on the free cell surface
Biophys. J. 2001. 80: 2667-2677.