研究業績

Publications

原著論文 総説・特許 講演Google Scholar, English

2016

46. #Baba M, #Iwamoto K, Iino R, Ueno H, Hara M, Nakanishia A, Kishikawa J, *Noji H, *Yokoyama K (#Equal contribution)

Rotation of artificial rotor axles in rotary molecular motors
PNAS 2016 113: 11214-11219. DOI: 10.1073/pnas.1605640113

 

45. Nakamura A, Tasaki T, Ishiwata D, Yamamoto M, Okuni Y, Visootsat A, Maximilien M, Noji H, Uchiyama T, Samejima M, Igarashi K, *Iino R

Single-molecule imaging analysis of binding, processive movement, and dissociation of cellobiohydrolase Trichoderma reesei Cel6A and its domains on crystalline cellulose
J. Biol. Chem. 2016 291: 22404-22413. DOI: 10.1074/jbc.M116.752048

Featured on the cover!

 

44. #Isojima H, #Iino R, Niitani Y, Noji H, *Tomishige M (#Equal contribution)
Direct observation of intermediate states during the stepping motion of kinesin-1
Nat Chem Biol 2016 12: 290-297. DOI: 10.1038/nchembio.2028

News and Views in Nature Chemical Biology: Motor proteins: Kinesin's gait captured

ブレスリリース「二本足で歩く分子モータータンパク質キネシンの足の動きを精細に可視化」

 

43. *Matsumoto Y, Sakakihara S, Grushnikov A, Kikuchi K, Noji H, Yamaguchi A, Iino R, Yagi Y, Nishino K
A microfluidic channel method for rapid drug-susceptibility testing of Pseudomonas aeruginosa
PLOS ONE 2016. 11(2): e0148797. DOI: 10.1371/journal.pone.0148797

 

2015

42. Obayashi Y, Iino R, *Noji H
A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate
Analyst 2015. 140: 5065-73. doi: 10.1039/c5an00714c

 

41. Enoki S, Iino R, Niitani Y, Minagawa Y, Tomishige M, *Noji H
High-speed angle-resolved imaging of single gold nanorod with microsecond temporal resolution and one-degree angle precision
Anal. Chem. 2015 87: 2079-2086. doi: 10.1021/ac502408c

 

40. Yukawa A, Iino R, Watanabe R, Hayashi S, *Noji H
Key chemical factors of arginine finger catalysis of F1-ATPase clarified by an unnatural amino acid mutation
Biochemistry. 2015 54: 472–480. doi: 10.1021/bi501138b

 

2014

39. #Ueno H, #Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Noji H, *Murata T, *Iino R  (#Equal contribution)
Torque generation of Enterococcus hirae V-ATPase
J. Biol. Chem. 2014 289: 31212-31223. doi: 10.1074/jbc.M114.598177

 

38. Ikeda T, Tsukahara T, Iino R, Takeuchi M, *Noji H
Motion capture and manipulation of single synthetic molecular rotors by optical microscopy
Angew. Chem. Int. Ed. 2014 53: 10082–10085. DOI: 10.1002/anie.201403091

プレスリリース: 1ナノメートルの人工分子マシン1個を「見て、触る」ことに成功:光学顕微鏡による 1分子モーションキャプチャ

Will be featured on the back cover!

 

37. Ikeda T, Iino R, *Noji H
Real-time fluorescence visualization of slow tautomerization of single free-base phthalocyanines under ambient conditions
Chem. Commun. 2014 50: 9443-9446. DOI: 10.1039/C4CC02574A

マイナビニュース記事. Introduced in UTokyo Research! (English, Japanese)
Real-time visualization of slow tautomerization in a single organic chromophore : Candidate for a 1-nanometer-sized molecular memory
有機色素分子1個の遅い互変異性化をリアルタイムに記録:分子メモリ開発につながる可能性
Featured on the front cover!

 

36. Shibafuji Y, Nakamura A, Uchihashi T, Sugimoto N, Fukuda S, Watanabe H, Samejima M, Ando T, Noji H, Koivula A, Igarashi K, *Iino R
Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII
J. Biol. Chem. 2014 289: 14056-14065. doi: 10.1074/jbc.M113.546085

 

35. Takehara H, Miyazawa K, Noda T, Sasagawa K, Tokuda T, Kim SH, Iino R, Noji H, *Ohta J
A CMOS image sensor with stacked photodiodes for lensless observation system of digital enzyme-linked immunosorbent assay
Jpn. J. Appl. Phys. 2014 53: 04EL02. doi: 10.7567/JJAP.53.04EL02

 

2013

34. #Minagawa Y, #Ueno H, Hara M, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Yamato I, Muneyuki E, Noji H, *Murata T, *Iino R (#Equal contribution)
Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase
J. Biol. Chem. 2013 288: 32700-32707. doi: 10.1074/jbc.M113.506329
Featured on the front cover! Designed by SCIEMENT

 

33. Fukuda S, Uchihashi T, Iino R, Okazaki Y, Yoshida M, Igarashi K, and *Ando T
High-speed atomic force microscope combined with single-molecule fluorescence microscope
Rev. Sci. Instrum. 2013 84: 073706. doi: 10.1063/1.4813280

 

32. Watanabe R, Tabata KV, Iino R, Ueno H, Iwamoto M, Oiki S, *Noji H
Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force
Nat. Commun. 2013 4: 1631. DOI:10.1038/ncomms2631

 

2012

31. Enoki S, Iino R, Morone N, Kaihatsu K, Sakakihara S, Kato N, *Noji H
Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy
PLOS ONE 2012. 7: e49208. DOI:10.1371/journal.pone.0049208

 

30. Kim SH, Iwai S, Araki S, Sakakihara S, Iino R, *Noji H
Large-scale femtoliter droplet array for digital counting of single biomolecules
Lab Chip 2012. 12: 4986-4991. DOI: 10.1039/C2LC40632B

 

29. You H, Iino R, Watanabe R, *Noji H
Winding single-molecule double-stranded DNA on a nanometer-sized reel
Nucleic Acids Research 2012. 40: e151. doi: 10.1093/nar/gks651

 

28. *Iino R, Hayama K, Amezawa H, Sakakihara S, Kim SH, Matsumoto Y, Nishino K, Yamaguchi A, Noji H
A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array
Lab Chip 2012. 12: 3923-3929. DOI: 10.1039/C2LC40394C
Introduced as one of the Emerging Investigators in Lab on a Chip!

 

27. *Hayashi S, Ueno H, Shaikh AR, Umemura M, Kamiya M, Ito Y, Ikeguchi M, Komoriya Y, Iino R, Noji H
Molecular mechanism of ATP hydrolysis in F1-ATPase revealed by molecular simulations and single molecule observations.
J. Am. Chem. Soc. 2012. 134: 8447–8454. DOI:10.1021/ja211027m

 

26. Komoriya Y, Ariga T, Iino R, Imamura H, Okuno D, *Noji H
Principle role of the arginine finger in rotary catalysis of F1-ATPase
J. Biol. Chem. 2012. 287: 15134-15142. DOI: 10.1074/jbc.M111.328153

 

25. *Sasagawa K, Ando K, Kobayashi T, Noda T, Tokuda T, Kim SH, Iino R, Noji H, Ohta J
Complementary metal–oxide–semiconductor image sensor with microchamber array for fluorescent bead counting
Jpn. J. Appl. Phys. 2012. 51. 02BL01. DOI: 10.1143/JJAP.51.02BL01

 

24. #Watanabe R, #Okuno D, Sakakihara S, Shimabukuro K, Iino R, Yoshida M, *Noji H (#Equal contribution)
Mechanical modulation of catalytic power on F1-ATPase
Nat. Chem. Biol. 2012. 8: 86-92. doi: 10.1038/nchembio.715

 

2011

23. #Uchihashi T, #Iino R, *Ando T, *Noji H. (#Equal contribution)
High-speed atomic force microscopy reveals rotary catalysis of rotorless F1-ATPase
Science 2011. 333: 755-758. DOI: 10.1126/science.1205510
Perspective of the Science
News of the Chemistry World
Research Highlight of the Nature Nanotech.
News of the BioTechniques


©Science

 

22. *Matsumoto Y, Hayama K, Sakakihara S, Nishino K, Noji H, *Iino R, Yamaguchi A.
Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels
PLOS ONE 2011.  6: e18547.

 

2010

21. Okuno D, Iino R, and *Noji H.
Stiffness of γ subunit of F1-ATPase
Eur. Biophys. J. 2010. 39:1589-1596.

 

20. Watanabe R, Iino R, *Noji H.
Phosphate-release in F1-ATPase catalytic cycle follows ADP release.
Nat. Chem. Biol. 2010. 6: 814–820. doi:10.1038/nchembio.443

Introduced in News and Views!

 

19. *Hayashi K, Ueno H, Iino R, and *Noji H.
Fluctuation theorem applied to F1-ATPase
Phys. Rev. Lett. 2010. 104: 218103-1-218103-4.

 

18. Ueno H, Nishikawa S, Iino R, Tabata KV, Sakakihara S, Yanagida T, *Noji H.
Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution
Biophys. J. 2010. 98: 2014-2023.

 

17. Saita E, Iino R, Suzuki T, Feniouk BA, Kinosita K. Jr, *Yoshida M.
Activation and stiffness of the inhibited states of F1-ATPase probed by single-molecule manipulation
J Biol Chem. 2010. 285: 11411-11417.

 

16. Sakakihara S, Araki S, *Iino R, *Noji H.
A single-molecule enzymatic assay in a directly accessible femtoliter droplet array
Lab Chip. 2010. 10: 3355-3362.

 

2009

15. *Imamura H, Huynh Nhat KP, Togawa H, Saito K, Iino R, Kato-Yamada Y, Nagai,T, *Noji H.
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators
Proc. Natl. Acad. Sci. U.S.A. 2009. 106: 15651-15656.

 

14. Enoki S, Watanabe R, Iino R, and *Noji H.
Single-molecule study on the temperature-sensitive reaction of F1-ATPase with a hybrid F1 carrying a single β(E190D)
J. Biol. Chem. 2009. 284: 23169-23176.

 

13. *Iino R, Hasegawa R, Tabata KV, *Noji H.
Mechanism of inhibition by C-terminal α-helices of the ε subunit of Escherichia coli FoF1-ATP synthase
J. Biol. Chem. 2009. 284:17457-17464.

 

2008

12. Okuno D, Fujisawa R, Iino R, Hirono-Hara Y, Imamura H, *Noji H.
Correlation between the conformational states of F1-ATPase as determined from its crystal structure and single-molecule rotation
Proc. Natl. Acad. Sci. U.S.A. 2008. 105: 20722-20727.

 

11. Watanabe R, Iino R, Shimabukuro K, Yoshida M, *Noji H.
Temperature-sensitive reaction intermediate of F1-ATPase
EMBO rep. 2008. 9: 84-90.

 

2007

10. Suzuki KGN, Fujiwara TK, Sanematsu F, Iino R, Edidin M, and *Kusumi A.
GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1
J. Cell Biol. 2007. 177: 717-730.

 

2005

9. Iino R, Murakami T, Iizuka S, Kato-Yamada Y, Suzuki T and *Yoshida M.
Real-time monitoring of conformational dynamics of the epsilon subunit in F1-ATPase
J. Biol. Chem. 2005. 280: 40130-40134.

 

8. Makiyo H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, *Yokoyama K and *Iwata S.
Structure of a central stalk subunit F of Prokaryotic V-type ATPase/synthase from Thermus thermophilus
EMBO J. 2005. 24: 3974-3983.

 

7. Koyama-Honda I, Ritchie K, Fujiwara T, Iino R, Murakoshi H, Kasai RS, and *Kusumi A.
Fluorescence imaging for monitoring the colocalization of two single molecules in living cells
Biophys. J. 2005. 88: 2126-2136.

 

2004

6. Murase K, Fujiwara T, Umemura Y, Suzuki K, Iino R, Yamashita H, Saito M, Murakoshi H, Ritchie K, and *Kusumi A.
Ultrafine membrane Compartments for molecular diffusion as revealed by single molecule techniques
Biophys. J. 2004. 86: 4075-4093.

 

5. Murakoshi H, Iino R, Kobayashi T, Fujiwara T, Ohshima C, Yoshimura A, and *Kusumi A.
Single-molecule imaging analysis of ras activation in living cells
Proc. Natl. Acad. Sci. U.S.A. 2004. 101: 7317-7322.

 

2003

4. Suzuki T, Murakami T, Iino R, Suzuki J, Ono S, Shirakihara Y, and *Yoshida M.
FoF1-ATPase/synthase is geared to the synthesis mode by conformational rearrangement of epsilon subunit in response to proton motive force and ADP/ATP balance
J. Biol. Chem. 2003. 278: 46840-46846.

 

3. Nakada C., Ritchie K., Oba Y, Nakamura M, Hotta Y, Iino R, Kasai S, Yamaguchi K, Fujiwara T, and *Kusumi A.
Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization
Nat. Cell Biol. 2003. 5: 626-632.

 

2. Ike H, Kosugi A, Kato A, Iino R, Hirano H, Fujiwara T, Ritchie K, and *Kusumi A.
Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single molecule fluorescence video imaging
ChemPhysChem. 2003. 4: 620-626.

 

2001

1. Iino R, Koyama I, and *Kusumi A.
Single molecule imaging of green fluorescent protein in living cells: E-cadherin forms oligomers on the free cell surface
Biophys. J. 2001. 80: 2667-2677.