Understand and engineer biomolecular machines


Welcome to IINO group 日本語

Activity of life is supported by molecular machines made of proteins. Protein molecular machines are far superior to synthetic molecular machines [1, 2] in many aspects. We will unveil operation principles of protein molecular machines with state-of-the-art single-molecule methods based on optical microscopy. With the help of computer science, we will also engineer new, non-natural protein molecular machines to understand their design principles. Our ultimate goal is to control life with engineered protein molecular machines.

See Research page for detail.

[1] The Nobel Prize in Chemistry 2016 "The design and synthesis of molecular machines"
[2] Peplow M. Nature 2015 "The tiniest Lego: a tale of nanoscale motors, rotors, switches and pumps"


Join our group

We welcome highly-motivated PhD course students.

Contact: iino_at_ims.ac.jp (change _at_ to @)

October 1, 2017 Ex-intenship student, Akasit (Bank) joined our group as a MEXT Scholarship graduate student. Welcome again!


News from our group

[Recent publications]

November 25, 2017 Our review article on single-cell assay of drug-efflux activity of bacteria has been published in Methods in Molecular Biology.

October 24, 2017 Dr. Nakamura and Tasaki-kun's paper has been published in PCCP! Single-molecule fluorescence imaging analysis of chitinase A! Inside back cover!

August 3, 2017 Our review article has been pubished in BBA General Subjects.


[Events and presentations]

February 17-21, 2018 Iino give a presentation in Biophysical Society 62nd Annual Meeting@San Francisco

February 2, 2018 A junior high-school student, Shimomura-kun visited our lab. Please enjoy science!

January 31, 2018 Iida-kun gave the Master thesis presentation. Very good presentation!

January 23, 2018 Prof. Dr. Joachim Heberle(Freie Universität Berlin) visited us and gave lecture in IMS colloquium

January 11, 2018 Dr. Ryohei Yasuda (Scientific Director, Max Planck Florida)gave seminar at NIPS

December 15, 2017 Prof. Shigehiko Hayashi (Kyoto Univ) visited us and gave lecture in IMS colloquium

November 29, 2017 Prof. Mikihiro Shibata(Kanazawa Univ) and his lab member visited us for collabrative experiments.

November 28-30, 2017 Prof. Chun-Biu Li(Stockholm Univ) and his lab members visited us for discussion.

November 24, 2017 Iino visited Prof. Hiroshi Masuhara and gave a talk in National Chiao Tung University@Taiwan

November 11-12, 2017 Dr. Ando gave a talk in Biomedical Molecular Imaging 2017@Taipei

October 30, 2017 Iino gave a talk in NANOTEC@Pathum Thani, Thailand

September 22, 2017 Dr. Chia-Lung Hsieh (IAMS, Academia Sinica) visited us and gave CIMoS seminar!

September 19-21, 2017 Lab members and our collaborators gave 10 presentations in BSJ annual meeting

September 4, 2017 Iida-kun has been selected as IMS-SRA! Congratulations!

August 17-18, 2017 OIIB summer school was held.

July 23-28, 2017 Nakamura-san gave a talk in Gordon Research Conference@Andover, USA

July 16-20, 2017 Iino gave a talk in IUPAB2017@Edinburgh, UK

July 14, 2017 We had an OIIB BBQ party!

July 13, 2017 We had a farewell party for Veda! Curry patry!

May 17, 2017 We took a group photo.

May 8, 2017 Veda (IIT Kharagpur) joined our group as an internship student. Welcome!

April 26, 2017 Iino visited the lab of Dr. Chia-Lung Hsieh (IAMS, Academia Sinica)

April 26, 2017 Iino visited the lab of Dr. En-Te Hwu (Edwin) (IOP, Academia Sinica) AFM youtube

April 25, 2017 IMS welcome party for freshmen!

April 24-26, 2017 Iino gave a talk in Frontier Bioorganization Forum 2017 @Taipei, Taiwan

April 1, 20178 Dr. Jun Ando joined our group as assinstant professor.

March 10, 2017 We had a farewell party for Daiki. Happy graduation!

February 11-15, 2017 Iino gave a presentation in Biophysical Society 61st Annual Meeting@New Orleans, Louisiana, USA

January 21-22, 2017 Iino gave a talk in The 5th International Symposium on "Dynamical Ordering & Integrated Functions" @Tokyo, Japan. "Single-molecule dynamics of natural and engineered molecular motors"

December 13-14, 2016 Iino gave a talk in 17th RIES-Hokudai International Symposium @Sapporo, Japan. "Watching dynamic motions of biological molecular machines"

November 25-27, 2016 We presented our data in 54th Annual Meeting of the Biophysical Society of Japan

November 21-22, 2016 We had OIIB retreat. Ishiwata-kun got poster award!

November 8-11, 2016 Iino give a talk in MNC2016 @Kyoto, Japan

November 7, 2016 Mr. Akasit Visootsat joined our group again. Welcome back Bank!

October 3-6, 2016 Iino gave a talk in 4th Kanazawa Bio-AFM Workshop "Intermediate states during the stepping motion of kinesin-1 revealed by high-speed single-molecule imaging with gold nanoprobes" @Kanazawa, Japan

September 8, 2016 Prof. Bo Huang (UCSF) visited our lab.

August 26, 2016 Dr. Yi ZHANG (Univ. Tokyo) visited our lab.

August 19, 2016 Mr. Maximilien MORICE (Chimie ParisTech) finished internship program. Thank you Maxi!

August 19, 2016 Prof. Ian Liau and Prof. Hsin-Yun Hsu (National Chiao Tung University) visited our lab.

August 18-19, 2016 OIIB Summer School 2016 was held!

August 17, 2016 Dr. Huijuan You (MBI, Singapore) visited our lab.

July 29, 2016 Prof. Zev Bryant (Stanford) will give a talk in Yamate evening seminar! 「Engineering controllable biomolecular motors

July 1, 2016 OIIB BBQ party!

June 28, 2016 IMS welcome party!

June 15, 2016 Iino gave a presentation at BPS Meeting, Engineering Approaches to Biomolecular Motors: From in vitro to in vivo "Direct observation of intermediate states during the stepping motion of kinesin-1"

June 3, 2016 Iino gave a talk in the symposium at Kasetsart University, Thailand "Biomass decomposition by cellulase observed at the single-molecule level"

April 8, 2016 We took a group photo!

April 1, 2016 Kawai-san and Iida-kun joined as members of our group!

March 29, 2016 We had a welcome party for Internship students from Chimie ParisTech!

March 22, 2016 Maximilien MORICE joined our group as an intenship student from Chimie ParisTech!

March 2, 2016 Drs. Thomas Meier (Imperial College London) and Takeshi Murata (Chiba Univ.) visited our lab!

February 15-17, 2016 Korea-Japan Seminars on Biomolecular Sciences was held at OCC, Okazaki. Program

February 12, 2016 Iino visited Dr. Thanit Praneenararat lab at Department of Chemistry, Faculty of Science, Chulalongkorn University. Ishiwata-kun will stay his lab and do collaborative experiments until the end of Feb.

February 10, 2016 Iino gave a talk in PACCON 2016 (Bangkok, Thailand) "Single-molecule analysis of new molecular motors hydrolyzing crystalline polysaccharides"

February 15-17, 2016 Korea-Japan Seminars on Biomolecular Sciences will be held at Okazaki Conference Center.

January 29, 2016 We had a farewell party for Bank, an internship student from Thailand! Thank you Bank!

January 28, 2016 Nakamura-san got the Guiness World Regords on the highest resolution (0.64Å) of X-ray crystallography of enzymes!

January 12, 2016 We went Thai restaurant in Okazaki!

December 16, 2015 Iino gave a talk in a symposium of Pacifichem 2015, Hawaii. "Dynamics of linear and rotary molecular motors revealed by gold nanoprobes"

December 9-10, 2015 OIIB retreat has been held! Lab members gave oral and poster presentations!

December 7, 2015 We had a year-end party with IMS-IIPA students!

November 2, 2015 Review article has been published!"Single-cell detection and collection of persister bacteria in a directly accessible femtoliter droplet array"

October 17, 2015 We had a Open Campus!

October 8, 2015 Iino will give a talk at IMS Workshop "Single-molecule analysis of energy conversion mechanism of molecular motor"

September 25, 2015 Minadawa-san got Ph. D from the Universiy of Tokyo.! Congratulations!

September 16, 2015 Mr. Paul Millman, the president of Chroma Technology visited us! Iino's old friend!

September 12-14, 2015 Lab members and collaborators gave 12 presentations in The 53rd Annual Meeting of the Biophysical Society of Japan!

September 4, 2015 We had a welcome party for students of IMS-IIPA! Takoyaki party!

September 1, 2015 Mrs. Okuni and Mr. Visootsat joined our group! Welcome!

August 12-13, 2015 OIIB held the summer school "Development of Biosensing Research"! We had a party with students who attended the school!

August 2-7, 2015 Nakamura-san gave a talk in the Gordon Research Conference!

July 24, 2015 We had a BBQ party!

July 17, 2015 Tokairin-san retired. Thank for everything!

July 17, 2015 Bottles of beer are prepared in the lab!

July 17, 2015 Koga-san organized lunch parety in CIMoS! Nakamura-san introduced himself.

July 10, 2015 Iino gave a talk in KAKENHI International Symposium on "Studying the Function of Soft Molecular Systems"

Jun 12-13, 2015 We held IMS Asian International Symposium "Supramolecular Dynamics at the Interface of Chemistry and Biology." All pictures are here!

June 1, 2015 Obayashi-san's paper has been published online in Analyst! Single-molecule digital enzyme assay of ALP!

May 17-24, 2015 Iino visited Malaysia, Thailand and Singapore for interview of IMS-IIPA. I met my previous student, You-san, again in Singapore!

May 9-12, 2015 Minagawa-san gave a poster presentation at ABA2015 held at Hangzhou, China!

April 30, 2015 Uchiyama-san and Tachioka-san from U-Tokyo and Tsubaki-san from JAMSTEC visited us for the collaborative experiments! Went a curry restaurant for dinner!

Apr 29, 2015 Iino was appointed as an Associate Editor of Frontiers in Bioengineering and Biotechnology.

Apr 17, 2015 We had a welcome party!

Apr 16, 2015 Mrs. Yamamoto and Mrs. Tokairin joined our group! Welcome!

Apr 14, 2015 We had okonomiyaki and yakisoba party!

Apr 1, 2015 Mrs. Nakane and Mr. Ishiwata joined our group! Welcome!

Mar 30-31, 2015 Iino gave a talk in IMS symposium "Architecture, dynamics, and functionality of molecular biosystems"
Mar 19, 2015 Our review article has been published in Current Opinion in Structural Biology!

Feb 9, 2015 Mr. Minagawa presented his results on V-ATPase in Biophysical Society 59th Annual Meeting!

Feb 3, 2015 Dr. Enoki's paper has been publisherd in Analytical Chemistry!

Jan 24, 2015 Iino visited and gave a talk at Department of Chemistry, Chulalongkorn University, Thailand.

Jan 24, 2015 Iino gave a talk in PACCON2015 "Watching dynamic motions of individual molecular motors with gold nanoprobes"
Jan 7, 2015 New year party at the lab!

Jan 1, 2015 A happy new year!

Dec 31, 2014 We published 6 original papers, 1 review article, and 3 Japanese reviews articles in 2014.

Dec 22, 2014 Miss Yukawa's paper has been published in Biochemistry!

Nov 27, 2014 Iino gave a talk in The 7th Korea-Japan Seminars on Biomolecular Sciences. (Poster 1.3MB)

Nov 21, 2014 We had a party with Prof. Shuhei Furukawa (Kyoto Univ.)!

Sep 26, 2014 Dr. Ueno's and Mr. Minagawa's paper has been published in JBC! "Torque generation of Enterococcus hirae V-ATPase"

Sep 19, 2014 Review article has been published in IUBMB Life! "Molecular structure and rotary dynamics of Enterococcus hirae V1-ATPase"
Sep 10, 2014 Iino gave a talk in ISSPIC XVII "Motions of individual biomolecular motors probed by gold nanoparticle and nanorod"

Aug 27-28, 2014 Summer school has been held at OIIB. "Biological Time and Biological Space"
Aug 1, 2014 Iino gave a talk in Andor Academy Kyoto "Single-molecule high-speed imaging of rotary and linear molecular motors"

Jul 3, 2014 Dr. Ikeda's paper has been published in Angew. Chem. Int. Ed.! Back cover!

Jun 2-3, 2014 Mr. Mniagawa and Mr. Tasaki gave presentatons at Tokyo ATPase Workshop!
Jun 1, 2014 Iino gourp at OIIB and IMS has been launched!