Research

研究内容

1. Understand biomolecular motors 日本語

We will unveil operation principles of protein molecular motors. Molecular motors generate mechanical forces and torques that drive their unidirectional motions from the energy of chemical reaction or the potential energy. We are studying linear [1-5] and rotary molecular motors [6-8]. Especially, we focus on new molecular motors such as chitinases, cellulases and V-ATPases. Our study is based on state-of-the-art single-molecule techniques. We are developing new single-molecule methods such as high resolution measurements with plasmonic nanoprobes [1, 4, 7-10]. We also tightly collaborate with Prof. Takayuni Uchihashi (Nagoya Univ.) for single-molecule measurement with high-speed AFM [11, 12] and development of hybrid single-molecule instruments [13]. We also determine crystal structures of biomolecular machines by X-ray crystallography [1].

 

[1] Nakamura A Nature Commun. 2018

[2] Nakamura A PCCP 2018 (Backcover

[3] Nakamura A J. Biol. Chem. 2016 (Cover

[4] Isojima H Nat. Chem. Biol. 2016

[5] Shibafuji Y J. Biol. Chem. 2014

[6] Tsunoda J Scientific Reports 2018

[7] Ueno H J. Biol. Chem. 2014

[8] Minagawa Y J. Biol. Chem. 2013 (Cover

[9] Ando J Biophys J 2018

[10] Enoki S Anal. Chem. 2015

[11] Uchihashi T Nature Commun. 2018

[12] Uchihashi T Science 2011 (Introduced in Nature Japan)

[13] Fukuda S Rev. Sci. Instrum. 2013

2. Engineer biomolecular motors

We will engineer non-natural biological molecular motors with new functions to understand their design principles [14-16]. Many biomolecular motors have similar structures, implying same evolutional origin. AAA+ family proteins are one of the representatives. Rotary molecular motors V1-ATPase and F1-ATPase also belong to this family [17, 18].

 

[14] Kawai F ACS Omega 2018

[15] Baba M PNAS 2016

[16] Yukawa A Biochemistry 2015

[17] Iino R Curr. Opin. Struct. Biol. 2013

[18] Iino R Curr. Opin. Struct. Biol. 2015