Department of Life and Coordination-Complex Molecular Science

FURUTANI, Yuji
FURUTANI, Yuji
 

Investigation of Molecular Mechanisms of Transporters and Receptors in Cell Membrane

Membrane proteins are important for homeostasis of living cells, which work as ion channel, ion pump, various types of chemical and biophysical sensors, and so on. These proteins are considered as one of important targets for biophysical studies. However, their molecular mechanisms have not been studied well, because X-ray crystallography and NMR spectroscopy are hard to access them in general. Our main goal is to clarify molecular mechanisms of transporters and receptors in cell membrane mainly by using stimulus-induced difference infrared spectroscopy which is sensitive to the structural and environmental changes of organic and bio-molecules.


(left) Schematic structure of whole V-ATPase protein complex (center) Sodium ion binding induced difference infrared spectrum (Y. Furutani et al., J. Am. Chem. Soc. 2011). The green and cyan colored bands are assigned to protonated and deprotonted carboxylic acid residues of Glu139 in K-ring, respectively. (right) Sodium ion binding site revealed by X-ray crystallography on K-ring.

(left) Schematic structure of whole V-ATPase protein complex (center) Sodium ion binding induced difference infrared spectrum (Y. Furutani et al., J. Am. Chem. Soc. 2011).
The green and cyan colored bands are assigned to protonated and deprotonted carboxylic acid residues of Glu139 in K-ring, respectively. (right) Sodium ion binding site revealed by X-ray crystallography on K-ring.


FURUTANI Group [Myodaiji Area]

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FURUTANI Group


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